Hi Guys
Heat will speed up the reaction but in reality your prespary won't be very hot after a few mins in the bottle so doesn't really make much difference.
Remember the often forgotten important factor is time, detergents, colloids, enzymes etc will gradualy wet out and break down the dirt, so good planning is needed.
Agitaion will also speed up wetting etc but I find using water hot, removes the need for this on most occasions.
Cheers
Doug